⮝ Full datasets listing
PXD060655
PXD060655 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Peritoneal fluid proteome in horses with colic |
| Description | Peritoneal fluid samples were obtained from horses presenting to the Veterinary Teaching Hospital (VTH) with signs of colic. Horses ≥ 2 years of age with no abdominal surgery in the previous 30 days were eligible for inclusion. PF samples were collected by abdominocentesis following standard clinical protocols into sterile blood collection tubes and aliquoted prior to storage at -80°C. Signalment, history, clinical evaluation parameters, diagnosis, treatment, complications, and short-term survival were recorded for each horse treated surgically for colic. After case resolution (discharge or death/euthanasia), horses were assigned to a group (non-strangulating or strangulating disease). Proteins were extracted by chloroform-methanol precipitation followed by digestion with trypsin. Prior to LC-MS analysis, the peptide amount was normalized according to a BCA assay. Dried peptides were suspended in 5% acetonitrile (ACN) with 0.1% formic acid (FA), and 1μg of peptides from each sample injected into an UltiMate 3000 RSLC nanoflow system coupled to a Q Exactive HF-X mass spectrometer (Thermo Scientific, Wilmington, DE). Protein identification and quantitation were performed in MaxQuant v2.010 software using the Uniprot Equus caballus, Bos Taurus, Homo sapiens and common lab contaminants databases. Expressed proteins were annotated with known or predicted functions using UniProtKB/Swiss-Prot. |
| HostingRepository | jPOST |
| AnnounceDate | 2026-02-11 |
| AnnouncementXML | Submission_2026-02-10_07:00:06.162.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Rebecca Bishop |
| SpeciesList | scientific name: cellular organisms; NCBI TaxID: 131567; |
| ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; deamidated L-glutamine; deamidated L-asparagine |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-02-10 15:06:31 | ID requested | |
| ⏵ 1 | 2026-02-10 07:00:06 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: horse, peritoneal fluid, label-free |
Contact List
| Annette M. McCoy | |
|---|---|
| lab head | |
| Rebecca Bishop | |
| contact affiliation | University of Illinois at Urbana-Champaign |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003601/ |
