PXD060108 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mass spectrometry of co-immunoprecipitation using Arabidopsis MKIP1-YFP as bait |
| Description | Arabidopsis thaliana (Arabidopsis) MATURASE K INTERACTING PROTEIN1 (MKIP1) is a plastid-localized, non-canonical member of the starch-branching enzyme family. Here we used co-immunoprecipitation (IP) coupled with proteomics to identify proteins bound to MKIP1 in stably transformed Arabidopsis lines expressing MKIP1 fused to a C-terminal YFP tag in the wild-type (Col-0) background. Untransformed wild-type (Col-0) Arabidopsis plants served as control. Proteins co-precipitating with MKIP1-YFP were purified via anti-GFP beads and identified via shotgun proteomics. We performed three individual experiments each using multiple replicates. Each experiment was separately analysed using MaxQuant. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-10 |
| AnnouncementXML | Submission_2026-02-10_06:45:05.996.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Barbara Pfister |
| SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-01-23 04:32:47 | ID requested | |
| ⏵ 1 | 2026-02-10 06:45:06 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Arabidopsis, Maturase K,LC-MS/MS, co-immunoprecipitation, MKIP1 |
Contact List
| Samuel C. Zeeman |
|---|
| contact affiliation | Institute of Molecular Plant Biology, Department of Biology, ETH Zurich, Switzerland |
| contact email | szeeman@ethz.ch |
| lab head | |
| Barbara Pfister |
|---|
| contact affiliation | ETH Zurich |
| contact email | bpfister@ethz.ch |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD060108 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD060108
- Label: PRIDE project
- Name: Mass spectrometry of co-immunoprecipitation using Arabidopsis MKIP1-YFP as bait
