PXD060055

PXD060055 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry of co-immunoprecipitation using tobacco Maturase K as bait
Description	Chloroplast group IIA introns derive from bacterial ribozymes. Their splicing likely requires Maturase K (MatK), which has been largely inaccessible to functional analyses being itself a chloroplast intron-encoded protein. Here we used co-immunoprecipitation coupled with proteomics to identify proteins bound to MatK in tobacco (Nicotiana tabacum) seedlings. We used homoplastomic tobacco lines modified to express NtMatK-HA (line NtMatK C+) or control lines carrying only the selection marker (line NtMatK C-). Proteins co-precipitating with NtMatK-HA were purified via anti-HA beads and identified via shotgun proteomics (n=3 seedling pools). The mass spectrometry data were further processed using Philosopher followed by label-free quantification based on precursor intensities using IonQuant and statistical analysis using Amica.
HostingRepository	PRIDE
AnnounceDate	2026-02-10
AnnouncementXML	Submission_2026-02-10_06:17:41.939.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Barbara Pfister
SpeciesList	scientific name: Nicotiana tabacum (Common tobacco); NCBI TaxID: NEWT:4097;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-22 02:15:40	ID requested
⏵ 1	2026-02-10 06:17:42	announced

Dataset with its publication pending

submitter keyword: LC-MS/MS, tobacco, co-immunoprecipitation, seedilings, chloroplasts

Samuel C. Zeeman
contact affiliation	Institute of Molecular Plant Biology, Department of Biology, ETH Zurich, Switzerland
contact email	szeeman@ethz.ch
lab head
Barbara Pfister
contact affiliation	ETH Zurich
contact email	bpfister@ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD060055

PRIDE project URI

• PRIDE
  1. PXD060055
     1. Label: PRIDE project
     2. Name: Mass spectrometry of co-immunoprecipitation using tobacco Maturase K as bait