PXD059966

PXD059966 is an original dataset announced via ProteomeXchange.

Title	Chaperone-mediated autophagy sustains muscle stem cell regenerative function
Description	Proteostasis supports stemness and its loss correlates with the functional decline of diverse stem cell types. Here we identify that chaperone-mediated autophagy (CMA), a selective autophagy pathway, is necessary for the regenerative capacity of muscle stem cells (MuSCs) throughout life. We show that CMA is active in MuSCs, while genetic loss of CMA in MuSCs or failure of CMA in normally aged cells causes a proliferative impairment, resulting in defective skeletal muscle regeneration. Reactivation of CMA in old MuSCs boosts their proliferative capacity and improves their regenerative ability. Using comparative proteomics to identify CMA substrates, we uncovered actin cytoskeleton and glycolytic metabolism as key processes altered in aged mice and human MuSCs. Our findings reveal CMA to be a decisive stem-cell-fate regulator, with implications for fostering muscle regeneration in old age.
HostingRepository	PRIDE
AnnounceDate	2025-10-17
AnnouncementXML	Submission_2025-10-17_01:15:52.391.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emilio Camafeita
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-20 03:18:46	ID requested
⏵ 1	2025-10-17 01:15:52	announced

Dataset with its publication pending

submitter keyword: CMA, Lamp2a, Muscle Stem Cells

Pura Muñoz-Cánoves
contact affiliation	Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain
contact email	pmunozcanoves@altoslabs.com
lab head
Emilio Camafeita
contact affiliation	Proteomics Unit Vascular Pathophysiology Area CNIC
contact email	ecamafeita@cnic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD059966

PRIDE project URI

• PRIDE
  1. PXD059966
     1. Label: PRIDE project
     2. Name: Chaperone-mediated autophagy sustains muscle stem cell regenerative function