PXD059641

PXD059641 is an original dataset announced via ProteomeXchange.

Title	Pervanadate reveals a new redox model of cellular phosphotyrosine 1 signaling C4PR_LIV
Description	Protein tyrosine phosphorylation (pTyr) is controlled by protein tyrosine kinases and phosphatases (PTPs) and allows cells to sense and respond to changes in their environment. Pervanadate is a widely used chemical tool that induces global pTyr, a phenomenon attributed to its properties as a PTP inhibitor, leading to the pervasive view that PTPs are the primary regulators of pTyr in cell culture. Here, we questioned this assumption and reveal that pervanadate not only inhibits PTPs, but also directly activates the SRC tyrosine kinase by oxidizing specific cysteine residues. Ourstudy provides mechanistic insight into how cysteine oxidation relieves the autoinhibited state of SRC and alters phosphopeptide binding. We further demonstrate that these cysteine residues are required for SRC to drive cellular overgrowth.
HostingRepository	PRIDE
AnnounceDate	2026-03-10
AnnouncementXML	Submission_2026-03-10_05:43:32.455.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD059641
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	leonard daly
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-10 08:26:37	ID requested
⏵ 1	2026-03-10 05:43:33	announced

Mulholland KE, Bourguet M, Cheng N, Rahman O, Ezeri, ņ, a D, Daly LA, Lai T, Aldaz Casanova S, Featherston T, Creixell P, Eyers CE, Messens J, Eyers PA, Byrne DP, Sharpe HJ, Pervanadate-induced oxidation relieves autoinhibition of the protein tyrosine kinase SRC. Sci Signal, 19(921):eady9437(2026) [pubmed]

10.1126/scisignal.ady9437;

10.6019/PXD059641;

submitter keyword: None

Prof Claire
contact affiliation	Centre for Proteome Research, Institute of Systems, Molecular and Integrative Biology, University of Liverpool; Liverpool, UK
contact email	ceyers@liverpool.ac.uk
lab head
leonard daly
contact affiliation	University of Liverpool
contact email	leonard.daly@liverpool.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD059641

PRIDE project URI

• PRIDE
  1. PXD059641
     1. Label: PRIDE project
     2. Name: Pervanadate reveals a new redox model of cellular phosphotyrosine 1 signaling C4PR_LIV