PXD058542

PXD058542 is an original dataset announced via ProteomeXchange.

Title	Activated gold-decorated nanoparticles enhance PD-1 blockade and promote cytotoxic T cell anti-tumor responses in cholangiocarcinoma
Description	Immunotherapy has revolutionized cancer treatment over the past decade. However, in most solid tumors, its effectiveness is often impaired owing to immune-resistance mediated through the tumor microenvironment (TME). In fibrotic cancers such as cholangiocarcinoma (CCA), the extracellular matrix (ECM) and cancer-associated fibroblasts (CAFs) create a dense, rigid stroma that hinders immune cell infiltration and fosters immunosuppression. Overcoming these physical and biological barriers is critical to fully unleash the potential of immunotherapeutic approaches. Here, the combination of photothermal therapy (PTT) with gold-iron oxide nanoflowers (GIONFs) effectively reshapes the TME by reducing ECM stiffness and facilitating immune cell infiltration. The GIONF-mediated TME mechanical reprogramming in combination with PD-1 immune checkpoint blockade leads to T-cell activation and reduces the immunosuppressive CAF subset. In preclinical models, this combination approach significantly supported anti-tumor immune responses and improved tumor control. Our findings emphasize the therapeutic potential of reshaping the TME to overcome immunotherapy resistance in fibrotic tumors like CCA. Targeting both the ECM and immune checkpoints may therefore represent a promising strategy to improve the efficacy of immunotherapy against desmoplastic cancers.
HostingRepository	PRIDE
AnnounceDate	2025-10-17
AnnouncementXML	Submission_2025-10-17_09:57:13.043.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chiara guerrera
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-03 11:32:30	ID requested
⏵ 1	2025-10-17 09:57:14	announced

Minini M, Avveduto G, Becharef S, Meunier-Thaens M, Lekbaby B, Perez-Lazon M, Machado A, Guilbert T, Lesieur J, Renault G, Ceccacci S, Guerrera IC, Klein C, Augustin J, Bazzi R, Roux S, Pol J, Donnadieu E, Gazeau F, Fouassier L, Laser-activated nanoparticles rewire tumor microenvironment enhancing PD-1 blockade and T cell response in cholangiocarcinoma. Hepatology, ():(2025) [pubmed]

10.1097/hep.0000000000001545;

submitter keyword: Proteomics, Data Independent Acquisition, Cholangiocarcinoma

Chiara Guerrera
contact affiliation	Faculty of Medecine, University Paris Cité SFR Necker INSERM US24 160 rue de Vaugirard | 75015 Paris
contact email	chiara.guerrera@inserm.fr
lab head
Chiara guerrera
contact affiliation	Necker proteomics, INSERM
contact email	chiara.guerrera@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD058542

PRIDE project URI

• PRIDE
  1. PXD058542
     1. Label: PRIDE project
     2. Name: Activated gold-decorated nanoparticles enhance PD-1 blockade and promote cytotoxic T cell anti-tumor responses in cholangiocarcinoma