PXD056442

PXD056442 is an original dataset announced via ProteomeXchange.

Title	Molecular mechanism of β-arrestin 2 interaction with phosphorylated intracellular loop 3 of dopamine receptor D2
Description	G protein-coupled receptors (GPCRs) are essential signaling molecules involved in various physiological processes, with β-arrestins playing crucial roles in receptor desensitization, internalization, and G protein-independent signaling. Although the interaction between the phosphorylated C-terminal tail (C-tails) of a GPCR and the N-domain of an arrestin has been well studied, the mechanisms underlying arrestin binding to GPCRs with short C-tails and long intracellular loop 3 (ICL3) remain unclear. We provide novel insights into the structural and functional interaction between the phosphorylated ICL3 of the dopamine receptor D2 (D2R) and β-arrestin 2 (βarr2) using hydrogen/deuterium exchange mass spectrometry (HDX-MS), and other biochemical approaches. By identifying key phosphorylated residues and elucidating distinct βarr2 conformational changes, our findings highlight novel aspects of GPCR–arrestin interaction.
HostingRepository	PRIDE
AnnounceDate	2026-02-10
AnnouncementXML	Submission_2026-02-09_20:20:57.481.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kiae Kim
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	No PTMs are included in the dataset
Instrument	Xevo G2 Q-Tof; Xevo G2-XS QTof

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-02 03:33:21	ID requested
⏵ 1	2026-02-09 20:20:58	announced

Kim K, Ji JS, Kim HR, Choi Y, Lee HH, Inoue A, Chung KY, -arrestin 2 interaction with phosphorylated intracellular loop 3 of dopamine receptor D2. Commun Biol, 8(1):1181(2025) [pubmed]

10.1038/s42003-025-08649-w;

submitter keyword: Arrestin, Dopamine receptor D2, phosphorylated intracellular loop 3,Hydrogen/deuterium exchange mass spectrometry (HDX-MS)

Ka Young Chung
contact affiliation	School of Pharmacy, Sungkyunkwan University, South Korea
contact email	kychung2@skku.edu
lab head
Kiae Kim
contact affiliation	Sungkyunkwan University
contact email	kimkiae95@skku.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD056442

PRIDE project URI

• PRIDE
  1. PXD056442
     1. Label: PRIDE project
     2. Name: Molecular mechanism of β-arrestin 2 interaction with phosphorylated intracellular loop 3 of dopamine receptor D2