PXD056357

PXD056357 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Elucidating the Roles of TTP and SMAD2/3 in HEK293T through LC-MS Analysis
Description	To further investigate the mechanism underlying the transcriptional regulation of m6A modification associated genes by TTP. we performed immunoprecipitation using anti-FLAG antibody in HEK293T cells transfected with pHAGE-TTP plasmids (overexpression of TTP) and then used mass spectrometry to identify the potential binding partners of TTP. the interactions between TTP and SMAD2/3 were confirmed by using coimmunoprecipitation in HEK293T, The N-Zn and Zn-C domain of TTP was found to be pivotal for its interaction with SMAD2/3.
HostingRepository	PRIDE
AnnounceDate	2026-05-14
AnnouncementXML	Submission_2026-05-13_18:27:13.369.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	rui zhou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-29 06:55:34	ID requested
⏵ 1	2026-05-13 18:27:13	announced

Publication List

10.1371/JOURNAL.PPAT.1014007;

10.1371/JOURNAL.PPAT.1014007;

Keyword List

submitter keyword: TTP,LC-MS,SMAD2/3, HEK293T

submitter keyword: TTP,LC-MS,SMAD2/3, HEK293T

Contact List

rui zhou
contact affiliation	lab
contact email	ruizhou@whu.edu.cn
lab head
rui zhou
contact affiliation	wuhan university
contact email	ruizhou@whu.edu.cn
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD056357
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD056357

PRIDE project URI

Repository Record List

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