PXD055169

PXD055169 is an original dataset announced via ProteomeXchange.

Title	Cysteine-enabled Cleavability to Advance Cross-linking Mass Spectrometry for Global Analysis of Endogenous Protein-Protein Interactions
Description	We have employed the sulfoxide-based cleavability to establish a novel cysteine-based MS-cleavable XL-MS platform, enabling proteome-wide PPI analysis using non-cleavable heterobifunctional K-C linkers. The effectiveness of the newly established platform has been demonstrated using three commercially available and non-cleavable NHS ester-haloacetamide heterobifunctional cross-linkers to map in vivo PPIs from HEK 293 cells.
HostingRepository	PRIDE
AnnounceDate	2025-10-17
AnnouncementXML	Submission_2025-10-17_04:39:21.563.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fenglong Jiao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-24 08:22:24	ID requested
⏵ 1	2025-10-17 04:39:22	announced

Dataset with its publication pending

submitter keyword: Protein structure,Cross-linking mass spectrometry,AlphaFold2, Cysteine

Lan Huang
contact affiliation	University of California, Irvine
contact email	lanhuang@uci.edu
lab head
Fenglong Jiao
contact affiliation	University of California, Irvine
contact email	fenglonj@uci.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD055169

PRIDE project URI

• PRIDE
  1. PXD055169
     1. Label: PRIDE project
     2. Name: Cysteine-enabled Cleavability to Advance Cross-linking Mass Spectrometry for Global Analysis of Endogenous Protein-Protein Interactions