Huh7 cells with or without expression of Flag-INSIG1/2 were treated with or without high glucose for 2 h. An immunoprecipitation assay was performed using anti-Flag antibody, and immunoprecipitates of Flag-INSIG1 were eluted with Flag peptide, separated using SDS-PAGE and stained with Coomassie Brilliant Blue. Selected peptide hits of proteins associated with Flag-INSIG1/2.