PXD048518
PXD048518 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic analysis of gastric cancero patient-derived tissue ECM |
| Description | Gastric cancer (GC) is a highly heterogeneous disease regarding histologic features, genotypes, and molecular phenotypes. Here, we investigated extracellular matrix (ECM)-centric analysis, examining its association with histologic subtypes and patient prognosis in human gastric cancer. We performed quantitative proteomic analysis of decellularized GC tissues that uncovered tumorous ECM, highlighting intertumoral heterogeneity in ECM composition. We identified 20 tumor-enriched proteins including four glycoproteins, serpin family H Member 1 (SERPINH1), Annexin family (ANXA3/4/5/13), S100A family (S100A6/8/9), MMP14, and other matrisome-associated proteins. In addition to these overall tumor-specific ECM proteins, histopathological characteristics of GC revealed variations in ECM composition, with the poorly cohesive carcinoma not otherwise specified (PCC-NOS) subtype being distinctly demarcated from other histologic subtypes. Integrating ECM proteomics with single-cell RNA sequencing, we identified crucial molecular markers in the PCC-NOS-specific stroma. PCC-NOS-enriched matrisome proteins (PEMs) and gene expression signatures of adipogenic cancer-associated fibroblasts (CAFadi) were closely linked, both associated with adverse outcomes in GC. Using tumor microarray analysis, we confirmed the CAFadi surface marker, ATP binding cassette subfamily A member 8 (ABCA8), predominantly present in PCC-NOS tumors. Our ECM-focused approach in identifying stromal characteristics paves the way for studies to determine their utility as biomarkers for patient stratification, offering valuable insights for linking molecular and histologic features in GC. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-12-23 |
| AnnouncementXML | Submission_2025-12-23_10:39:16.803.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Yeji Yang |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-01-12 23:19:41 | ID requested | |
| ⏵ 1 | 2025-12-23 10:39:17 | announced |
Publication List
| Lee HJ, Kwak Y, Na YS, Kim H, Park MR, Jo JY, Kim JY, Cho SJ, Kim P, Proteomic Heterogeneity of the Extracellular Matrix Identifies Histologic Subtype-Specific Fibroblast in Gastric Cancer. Mol Cell Proteomics, 23(10):100843(2024) [pubmed] |
Keyword List
| submitter keyword: Gastric cancer, poorly cohesive carcinoma, Proteomics, Tumor microenvironment |
Contact List
| Jin Young Kim | |
|---|---|
| contact affiliation | Korea Basic Science Institute |
| contact email | jinyoung@kbsi.re.kr |
| lab head | |
| Yeji Yang | |
| contact affiliation | Korea Basic Science Institute |
| contact email | yangyj@kbsi.re.kr |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v06/MSV000093859/ |
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