PXD045648

PXD045648 is an original dataset announced via ProteomeXchange.

Title	Plasma membrane deformation by one-dimensional wetting triggers the formation of a branched F-actin network in endothelial cells
Description	Membrane deformation by one-dimensional (1D) wetting, a physical process driven by the interaction of the plasma membrane with adhesive nanofibers, triggers the formation of filopodium-like plasma membrane protrusions. In these protrusions, F-actin reorganization occurs after membrane deformation. Here, we interrogate the molecular mechanisms underlying F-actin reorganization in such protrusions formed by human primary epithelial and endothelial cells. While generally assumed that filopodium-like protrusions encompass F-actin bundles, as we observed in epithelial cells, cryo-electron tomography unexpectedly revealed a branched F-actin network in 1D wetting protrusions formed by endothelial cells. Combining high resolution photonic microscopy approaches with genetic and drug perturbations, we show that Arp2/3 activity is required for the proper reorganization of F-actin in 1D wetting endothelial protrusions, a process regulated through the local activation of Cdc42 and N-WASP. Our data suggest that F-actin reorganization in response to membrane deformation by 1D wetting is differentially regulated in epithelial versus endothelial cells, possibly impacting a wide range of physiological processes, such as cell migration, adhesion, and microenvironment probing.
HostingRepository	PRIDE
AnnounceDate	2026-05-13
AnnouncementXML	Submission_2026-05-13_12:53:04.940.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Magalie Duchateau
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Neisseria meningitidis serogroup C (strain 8013); NCBI TaxID: NEWT:604162;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-25 04:46:55	ID requested
⏵ 1	2026-05-13 12:53:05	announced

Dataset with its publication pending

submitter keyword: Plasma membrane deformation, branched F-actin network

Guilllaume Dumenil
contact affiliation	Institut Pasteur, Université Paris Cité, INSERM U1225, Pathogenesis of Vascular Infections unit, F-75015 Paris, France
contact email	guilllaume.dumenil@pasteur.fr
lab head
Magalie Duchateau
contact affiliation	Institut Pasteur
contact email	magalie.duchateau@pasteur.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD045648

PRIDE project URI

• PRIDE
  1. PXD045648
     1. Label: PRIDE project
     2. Name: Plasma membrane deformation by one-dimensional wetting triggers the formation of a branched F-actin network in endothelial cells