PXD037421

PXD037421 is an original dataset announced via ProteomeXchange.

Title	Protein SUMOylation in the Drosophila ovary
Description	Genome regulation involves complex and highly regulated protein interactions that are often mediated through post-translational modifications (PTMs). SUMOylation – the covalent attachment of the small ubiquitin-like modifier (SUMO) – is a conserved PTM in eukaryotes that has been implicated in a number of essential processes such as nuclear import, DNA damage repair, transcriptional control, and chromatin organization. In Drosophila, SUMO is essential for viability and its depletion from the female germline causes infertility associated with global loss of heterochromatin, and illicit upregulation of transposons and lineage-inappropriate genes. However, the specific targets of SUMO and its mechanistic role in different cellular pathways are still poorly understood. Here, we developed a proteomics-based strategy to characterize the SUMOylated proteome in Drosophila that allowed us to identify ~1500 SUMO sites in 843 proteins in the fly ovary. A high confidence set of SUMOylated proteins is highly enriched in factors involved in heterochromatin regulation and the piRNA pathway that represses transposons. Furthermore, SUMOylation of several piRNA pathway proteins occurs in a Piwi-dependent manner, indicating a functional implication of this modification in the cellular response to transposon activity. Together, these data highlight the impact of SUMOylation on epigenetic regulation and reveal an unexpectedly broad role of the SUMO pathway in the cellular defense against genomic parasites. Finally, this work provides a valuable resource and a system that can be adapted to the study of SUMOylation in other Drosophila tissues.
HostingRepository	PRIDE
AnnounceDate	2023-04-17
AnnouncementXML	Submission_2023-04-16_18:15:07.180.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MariaNinova
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	ubiquitinylated lysine
Instrument	1200 series LC/MSD VL

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-14 04:51:10	ID requested
⏵ 1	2023-04-16 18:15:07	announced

Dataset with its publication pending

submitter keyword: drosophila, SUMO, ovary

Maria AntoninovaNinova
contact affiliation	Department of Biochemistry, UC Riverside
contact email	mninova@ucr.edu
lab head
MariaNinova
contact affiliation	UC Riverside
contact email	mninova@ucr.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037421
     1. Label: PRIDE project
     2. Name: Protein SUMOylation in the Drosophila ovary