PXD031125

PXD031125 is an original dataset announced via ProteomeXchange.

Title	Hypoxia reveals the new function of Foxn1 in keratinocytes antioxidant defense system
Description	Skin exposed to environmental threats including injuries and oxidative stress developed an efficient but not fully recognized systems of repair and antioxidant protection. In this project we provide evidence that Foxn1 in keratinocytes regulates elements of electron transport chain and participates in thioredoxin system (Txn2, Txnrd3, Srxn1) induction, particularly under hypoxic environment. It is shown that Foxn1 in keratinocytes upregulate glutathione thioredoxin reductase 3 (Txnrd3) protein, and high levels of Txnrd3 mRNA were detected in injured skin of Foxn1+/+ mice. It also shown that Foxn1 strongly downregulate CCN2 protein participating in reconstruction of the epidermis after injury. As in vitro assay revealed that Foxn1 controls keratinocytes migration stimulating it under normoxia and suppressing under hypoxia. Keratinocytes overexpressing Foxn1 and exposed to hypoxia showed reduced ability to promote angiogenesis by downregulation of Vegfa. In conclusion, this study showed a new mechanism in which Foxn1, along with hypoxia, participates in the activation of antioxidant defense and controls functional properties of keratinocytes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:38:12.127.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mirva Pääkkönen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-20 09:31:16	ID requested
1	2022-07-07 21:31:55	announced
2	2023-11-14 08:55:07	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:38:12	announced	2024-10-22: Updated project metadata.

10.1096/FJ.202200249RR;

submitter keyword: Thioredoxin system,Skin, Foxn1, Keratinosytes, Hypoxia

Barbara Gawrońska-Kozak
contact affiliation	Institute of Animal Reproduction and Food Research, Polish Academy of Sciences in Olsztyn, Poland
contact email	b.kozak@pan.olsztyn.pl
lab head
Mirva Pääkkönen
contact affiliation	Turku Bioscience Centre
contact email	mirva.paakkonen@bioscience.fi
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD031125

PRIDE project URI

• PRIDE
  1. PXD031125
     1. Label: PRIDE project
     2. Name: Hypoxia reveals the new function of Foxn1 in keratinocytes antioxidant defense system