PXD030974

PXD030974 is an original dataset announced via ProteomeXchange.

Title	Proteomics approach unravels early changes in Human Fibro-blasts-Pancreatic Ductal Adenocarcinoma cells crosstalk
Description	Pancreatic ductal adenocarcinoma (PDAC) is a leading causes of cancer mortality worldwide. Non-specific symptoms, lack of biomarkers in the early stages, and drug resistance due to the presence of a dense fibrous stroma all contribute to poor outcome of this disease. The extracellular matrix of PDAC is secreted by activated fibroblasts known as cancer associated fibroblasts (CAFs). These fibroblasts can also affect cancer cell aggressiveness by paracrine crosstalk with cancer and immune cells present inside the tumor mass. The interplay among tumoral cells, immune cells and CAFs involve a huge array of molecules especially cytokines. Given the importance of CAFs in PDAC pathology it is critical to recognize the mechanisms involved in the crosstalk between these cells. To this aim, we first identified the proteins released from the pancreatic cancer cell line MIA-PaCa2 by proteomic analysis of their conditioned medium (CM). Second, fibroblasts were treated with MIA-PaCa2 CM for 24 and 48 hours and their proteostatic changes were detected by proteomics. Pathway analysis indicated that fi-broblasts treated with CM undergo changes compatible with the activation of migra-tion, vasculogenesis, cellular homeostasis and metabolism of ammino acids and re-duced apoptosis. These biological activities are possibly regulated by ITGB3 and TGFB1/2 in an early stage (24 hours) while SMAD3 and STAT3 could be the key player at later times (48 hours). In conclusion this study shed light on the crosstalk between PDAC cells and associated fibroblasts.
HostingRepository	PRIDE
AnnounceDate	2022-05-20
AnnouncementXML	Submission_2022-05-20_05:32:18.743.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maria Concetta Cufaro
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-14 08:33:02	ID requested
⏵ 1	2022-05-20 05:32:20	announced

Damiani V, Cufaro MC, Fucito M, Dufrusine B, Rossi C, Del Boccio P, Federici L, Turco MC, Sallese M, Pieragostino D, De Laurenzi V, Proteomics Approach Highlights Early Changes in Human Fibroblasts-Pancreatic Ductal Adenocarcinoma Cells Crosstalk. Cells, 11(7):(2022) [pubmed]

submitter keyword: Cancer associated fibroblasts

PDAC microenvironment

Proteomics

Ingenuity Pathway Analysis

Damiana Pieragostino
contact affiliation	Department of Innovative Technologies in Medicine and Dentistry,University “G. d’Annunzio” of Chieti-Pescara, Italy
contact email	damiana.pieragostino@unich.it
lab head
Maria Concetta Cufaro
contact affiliation	University "G. d'Annunzio" of Chieti
contact email	maria.cufaro@unich.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD030974

PRIDE project URI

• PRIDE
  1. PXD030974
     1. Label: PRIDE project
     2. Name: Proteomics approach unravels early changes in Human Fibro-blasts-Pancreatic Ductal Adenocarcinoma cells crosstalk