PXD023467

PXD023467 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Ago3 interaction proteins in BmN4 cell
Description	We prepared BmN4 cell lysates in Co-IP buffer [30 mM HEPES-KOH pH 7.3, 150 mM potassium acetate, 5 mM magnesium acetate, 5 mM dithiothreitol, 0.1% (w/v) NP-40 and cOmplete ULTRA EDTA-free protease inhibitor (Roche)] with and without 1 mM EDTA and conducted immunoprecipitation assays using anti-Ago3 antibody. The reaction was terminated by boiling with SDS sample buffer.
HostingRepository	jPOST
AnnounceDate	2021-01-08
AnnouncementXML	Submission_2022-09-18_02:32:04.642.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lumi Negishi
SpeciesList	scientific name: Bombyx mori; NCBI TaxID: 7091;
ModificationList	S-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-07 20:45:28	ID requested
1	2021-01-07 21:14:53	announced
2	2021-02-04 17:23:36	announced	2021-02-04: Updated FTP location.
3	2021-07-19 00:46:14	announced	2021-07-19: Updated PubMed.
⏵ 4	2022-09-18 02:32:05	announced	2022-09-18: Updated FTP location.

Publication List

Murakami R, Sumiyoshi T, Negishi L, Siomi MC, DEAD-box polypeptide 43 facilitates piRNA amplification by actively liberating RNA from Ago3-piRISC. EMBO Rep, 22(4):e51313(2021) [pubmed]

Murakami R, Sumiyoshi T, Negishi L, Siomi MC, DEAD-box polypeptide 43 facilitates piRNA amplification by actively liberating RNA from Ago3-piRISC. EMBO Rep, 22(4):e51313(2021) [pubmed]

Keyword List

submitter keyword: Ago3, BmN4, piRNA

submitter keyword: Ago3, BmN4, piRNA

Contact List

Mikiko C. Siomi
lab head
Lumi Negishi
contact affiliation	The University of Tokyo
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001051/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001051/