PXD014896

PXD014896 is an original dataset announced via ProteomeXchange.

Title	SCC1 interaction proteomics in Saccharomyces cerevisiae
Description	FACT mediates cohesin function on chromatin Cohesin is a key regulator of genome architecture with roles in sister chromatid cohesion and the organisation of higher-order structures during interphase and mitosis. The recruitment and mobility of cohesin complexes on DNA are restricted by nucleosomes. Here we show that cohesin role in chromosome organization requires the histone chaperone FACT. Depletion of FACT in metaphase cells affects cohesin stability on chromatin reducing its accumulation at pericentric regions and binding on chromosome arms. Using Hi-C, we show that cohesin-dependent TAD (Topological Associated Domains)-like structures in G1 and metaphase chromosomes are disrupted in the absence of FACT. Surprisingly, sister chromatid cohesion is intact in FACT-depleted cells, although chromosome segregation failure is observed. Our results uncover a role for FACT in genome organisation by facilitating cohesin dependent compartmentalization of chromosomes into loop domains.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:56:58.247.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Holger Kramer
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-02 07:46:09	ID requested
1	2019-10-03 06:17:56	announced
⏵ 2	2024-10-22 04:56:58	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: FACT complex, SCC1 interactomics, Saccharomyces cerevisiae

Jonay García-Luis
contact affiliation	MRC London Institute of Medical Sciences Hammersmith Hospital Campus Du Cane Road, London, W12 0NN United Kingdom
contact email	j.garcia-luis@lms.mrc.ac.uk
lab head
Holger Kramer
contact affiliation	MRC London Institute of Medical Sciences
contact email	h.kramer@lms.mrc.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014896
     1. Label: PRIDE project
     2. Name: SCC1 interaction proteomics in Saccharomyces cerevisiae