PXD013808

PXD013808 is an original dataset announced via ProteomeXchange.

Title	FAM83D directs protein kinase CK1α to the mitotic spindle for proper spindle positioning
Description	The concerted action of many protein kinases helps orchestrate the error-free progression through mitosis of mammalian cells. The roles and regulation of some prominent mitotic kinases, such as cyclin-dependent kinases, are well-established. However, these and other known mitotic kinases alone cannot account for the extent of protein phosphorylation that has been reported during mammalian mitosis. Here we demonstrate that CK1α, of the casein kinase 1 family of protein kinases, localises to the spindle and is required for proper spindle-positioning and timely cell division. CK1α is recruited to the spindle by FAM83D, and cells devoid of FAM83D, or those harbouring CK1α-binding-deficient FAM83DF283A/F283A knockin mutation, display pronounced spindle-positioning defects, and a prolonged mitosis. Restoring FAM83D at the endogenous locus in FAM83D-/- cells, or artificially delivering CK1α to the spindle in FAM83DF283A/F283A cells, rescues these defects. These findings implicate CK1α as new mitotic kinase that orchestrates the kinetics and orientation of cell division.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:56:28.663.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gopal Sapkota
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	dihydroxylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-13 05:53:25	ID requested
1	2019-07-15 01:08:52	announced
2	2019-08-01 04:47:29	announced	Updated publication reference for PubMed record(s): 31338967.
⏵ 3	2024-10-22 04:56:30	announced	2024-10-22: Updated project metadata.

10.15252/embr.201847495;

Fulcher LJ, He Z, Mei L, Macartney TJ, Wood NT, Prescott AR, Whigham AJ, Varghese J, Gourlay R, Ball G, Clarke R, Campbell DG, Maxwell CA, Sapkota GP, to the mitotic spindle for proper spindle positioning. EMBO Rep, 20(9):e47495(2019) [pubmed]

submitter keyword: protein kinase CK1α, mitotic spindle,FAM83D

Gopal P. Sapkota
contact affiliation	MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, UK
contact email	g.sapkota@dundee.ac.uk
lab head
Gopal Sapkota
contact affiliation	University of Dundee
contact email	g.sapkota@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD013808

PRIDE project URI

• PRIDE
  1. PXD013808
     1. Label: PRIDE project
     2. Name: FAM83D directs protein kinase CK1α to the mitotic spindle for proper spindle positioning