PXD013219

PXD013219 is an original dataset announced via ProteomeXchange.

Title	The Flemmingsome reveals an ESCRT-to-membrane coupling required for cytokinesis
Description	Cytokinesis requires the constriction of ESCRT-III filaments on the midbody side, where abscission occurs. After ESCRT recruitment at the midbody, it is not known how the ESCRT-III machinery localizes to the abscission site. We obtained the proteome of intact, post-abscission midbodies (Flemmingsome) and revealed enriched proteins in this organelle. We propose that the ESCRT-III machinery must be physically coupled to a membrane protein at the cytokinetic abscission site for productive scission, revealing novel common requirements in cytokinesis, exosome formation and retroviral budding.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:04:07.368.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibaut Douché
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	L-methionine sulfoxide; ubiquitination signature dipeptidyl lysine; phosphorylated residue; mono N-acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-25 07:18:12	ID requested
1	2020-05-21 23:54:20	announced
⏵ 2	2024-10-22 05:04:08	announced	2024-10-22: Updated project metadata.

Addi C, Presle A, Fr, é, mont S, Cuvelier F, Rocancourt M, Milin F, Schmutz S, Chamot-Rooke J, Douch, é T, Duchateau M, Giai Gianetto Q, Salles A, M, é, nager H, Matondo M, Zimmermann P, Gupta-Rossi N, Echard A, The Flemmingsome reveals an ESCRT-to-membrane coupling via ALIX/syntenin/syndecan-4 required for completion of cytokinesis. Nat Commun, 11(1):1941(2020) [pubmed]

10.1038/s41467-020-15205-z;

submitter keyword: Human, ESCRT machinery, Cytokinesis, Midbody, QExactive, LC-MS/MS

Arnaud Echard
contact affiliation	Membrane Traffic and Cell Division Lab, Institut Pasteur, UMR3691, CNRS, 25–28 rue du Dr Roux, F-75015 Paris, France
contact email	arnaud.echard@pasteur.fr
lab head
Thibaut Douché
contact affiliation	Institut Pasteur
contact email	thibaut.douche@pasteur.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD013219

PRIDE project URI

• PRIDE
  1. PXD013219
     1. Label: PRIDE project
     2. Name: The Flemmingsome reveals an ESCRT-to-membrane coupling required for cytokinesis