PXD009494

PXD009494 is an original dataset announced via ProteomeXchange.

Title	Yeast genetic codes - Endogenous Stochastic Decoding of the CUG Codon by Competing Ser- and Leu-tRNAs in Ascoidea asiatica
Description	While the “universal” genetic code is now known not to be universal, and stop codons can have multiple meanings, one regularity remains, namely that for a given sense codon there is a unique translation. Examining CUG usage in yeasts that have transferred CUG away from leucine, we here report the first example of dual coding: Ascoidea asiatica stochastically encodes CUG as both serine and leucine. This is deleterious as evidenced by CUG codons being rare, never at conserved serine/leucine residues and predominantly in lowly expressed genes. Related yeasts solve the problem by loss-of-function of one of the two tRNAs. This dual-coding is consistent with the tRNA-loss driven codon reassignment hypothesis and provides a unique example of a proteome that cannot be deterministically predicted.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:44:17.534.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Kollmar
SpeciesList	scientific name: Babjeviella inositovora NRRL Y-12698; NCBI TaxID: 984486; scientific name: Nakazawaea peltata; NCBI TaxID: 1538182; scientific name: Ascoidea rubescens DSM 1968; NCBI TaxID: 1344418; scientific name: Clavispora lusitaniae ATCC 42720; NCBI TaxID: 306902; scientific name: Saccharomycopsis malanga; NCBI TaxID: 44092; scientific name: Ascoidea asiatica; NCBI TaxID: 1301101; scientific name: Saccharomycopsis fibuligera; NCBI TaxID: 4944;
ModificationList	mono N-acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-13 02:19:21	ID requested
1	2018-06-13 07:40:28	announced
2	2018-06-20 00:20:17	announced	Updated publication reference for PubMed record(s): 29910077.
⏵ 3	2024-10-22 04:44:22	announced	2024-10-22: Updated project metadata.

M, ü, hlhausen S, Schmitt HD, Pan KT, Plessmann U, Urlaub H, Hurst LD, Kollmar M, Endogenous Stochastic Decoding of the CUG Codon by Competing Ser- and Leu-tRNAs in Ascoidea asiatica. Curr Biol, 28(13):2046-2057.e5(2018) [pubmed]

10.1016/j.cub.2018.04.085;

curator keyword: Biological

submitter keyword: Ascoidea, Clavispora, Saccharomycopsis, Nakazawaea, Babjeviella

Martin Kollmar
contact affiliation	Group Systems Biology of Motor Proteins, Department of NMR-based Structural Biology, Max-Planck-Institute for Biophysical Chemistry, Göttingen, Germany
contact email	mako@nmr.mpibpc.mpg.de
lab head
Martin Kollmar
contact affiliation	MPI for Biophysical Chemistry
contact email	mako@nmr.mpibpc.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009494
     1. Label: PRIDE project
     2. Name: Yeast genetic codes - Endogenous Stochastic Decoding of the CUG Codon by Competing Ser- and Leu-tRNAs in Ascoidea asiatica