PXD001039

PXD001039 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics of the Milk Fat Globule Membrane: new insights into lipid droplet secretion from the mammary epithelial cell
Description	Lipid droplets are secreted into milk by a complex process which begins by burgeoning oflipids at the endoplasmic reticulum of the mammary epithelial cell (MEC). Lipid dropletsenveloped by organelle-derived phospholipids reach the apical pole of the cell where they arewrapped by the plasma membrane to be secreted as fat globules into milk. Analyzing the fineprotein composition of the Milk Fat Globule Membrane (MFGM), the triple-layeredmembrane surrounding milk lipid droplets can therefore provide mechanistic clues to betterunderstand lipid droplet biosynthesis and secretion pathways. We combined a high sensitiveQ Exactive LC-MS/MS analysis of MFGM-derived peptides with the use of an in-house database intended to improve protein identification in the goat species. Using this approach, we performed the identification of 442 functional groups of proteins in the MFGM from goatmilk. To get a more comprehensive view of intracellular mechanisms driving lipid dropletdynamics in the MEC, we decided to investigate for the first time whether MFGM proteinswere phosphorylated. A phosphopeptide enrichment approach let us pinpoint 271 sites ofphosphorylation on 124 unique goat MFGM proteins. Enriched gene ontology termsassociated with phosphorylated MFGM proteins were protein transport and actin cytoskeletonorganization. Gained data are discussed with regard to lipid secretory mechanisms in the MEC.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:11:10.702.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	HENRY CELINE
SpeciesList	scientific name: Capra hircus (Goat); NCBI TaxID: 9925;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-06-06 02:43:23	ID requested
1	2015-05-12 02:12:10	announced
⏵ 2	2024-10-22 04:11:11	announced	2024-10-22: Updated project metadata.

10.1002/pmic.201400245;

Henry C, Saadaoui B, Bouvier F, Cebo C, Phosphoproteomics of the goat milk fat globule membrane: New insights into lipid droplet secretion from the mammary epithelial cell. Proteomics, 15(13):2307-17(2015) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: mammary epithelial cell, secretion, phosphoproteome,milk, lipid droplet

Christelle CEBO
contact affiliation	INRA, UMR 1313 Unité Génétique Animale animale et Biologie Intégrative
contact email	christelle.cebo@jouy.inra.fr
lab head
HENRY CELINE
contact affiliation	INRA
contact email	celine.henry@jouy.inra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/05/PXD001039

PRIDE project URI

• PRIDE
  1. PXD001039
     1. Label: PRIDE project
     2. Name: Phosphoproteomics of the Milk Fat Globule Membrane: new insights into lipid droplet secretion from the mammary epithelial cell