PXD000363

PXD000363 is an original dataset announced via ProteomeXchange.

Title	PG-regulated Synechocystis sp. PCC6803
Description	Cells from the Synechocystis sp. PCC6803 strain were used to identify phosphatidylglycerol-regulated proteins by label-free quantitative shotgun proteomics combining SDS-PAGE prefractionation and data-dependent LC–MS/MS. Acquired data was searched against a composite protein sequence database of cyanobacteria using the Mascot search algorithm. Protein identifications were accepted after rigorous validation criteria of Peptide Prophet, Protein Prophet and requiring at least two unique proteolytic peptides for each protein. Extracted peptide intensity features were used for the label-free comparison of differential protein expression in the mutant cyanobacteria cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_09:01:59.723.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000363
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Laszlo Prokai
SpeciesList	scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148; scientific name: Synechocystis sp.; NCBI TaxID: 1143;
ModificationList	sulfated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2013-07-25 01:20:00	ID requested
1	2014-03-06 03:27:32	announced
2	2014-07-28 00:42:33	announced	Updated project metadata.
3	2024-10-07 09:01:47	announced	2024-10-07: Updated project metadata.
⏵ 4	2024-10-07 09:02:00	announced	2024-10-07: Updated project metadata.

10.6019/PXD000363;

10.1002/pmic.201300372;

Talamantes T, Ughy B, Domonkos I, Kis M, Gombos Z, Prokai L, Label-free LC-MS/MS identification of phosphatidylglycerol-regulated proteins in Synechocystis sp. PCC6803. Proteomics, 14(9):1053-7(2014) [pubmed]

submitter keyword: Quantitative label-free proteomics,Cyanobacteria, Phosphatidylglycerol, GeLC-MS/MS, Synechocystis sp. PCC6803

Laszlo Prokai
contact affiliation	Department of Pharmacology & Neuroscience UNT Health Science Center Fort Worth, TX, USA
contact email	advancedms@unthsc.edu
lab head
Laszlo Prokai
contact affiliation	Department of Pharmacology & Neuroscience UNT Health Science Center Fort Worth, TX, USA
contact email	advancedms@unthsc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/03/PXD000363

PRIDE project URI

• PRIDE
  1. PXD000363
     1. Label: PRIDE project
     2. Name: PG-regulated Synechocystis sp. PCC6803