ulcerative colitis, colorectal cancer, Data-Independent Acquisition, bioinformatics analysis, Gene Ontology, Gene Set Enrichment AnalysisWe employed Data-Independent Acquisition (DIA)-based mass spectrometry to profile the proteomes of paired tissue and exudate samples from patients with UC and CRC. Differentially expressed proteins (DEPs) were analyzed using Gene Ontology (GO), KEGG, and Gene Set Enrichment Analysis (GSEA). Four candidate biomarkers, CDA, REG4, LCN2, and TNS1, were validated in serum samples using ELISA and receiver operating characteristic (ROC) curve analysis.