PXD082807

PXD082807 is an original dataset announced via ProteomeXchange.

Title	Proteomics of immortalized megakaryocytes fractionated by cell size and culture duration
Description	Quantitative label-free proteome analysis of iPSC-derived immortalized megakaryocytes (imMKCLs), sorted by cell size and culture duration into six fractions, was performed in this project. Following tryptic digestion, peptide samples were analyzed by liquid chromatography–mass spectrometry (LC-MS). For each run, 3 µg of peptide mixture was injected into an Ultimate 3000 nanoUHPLC system (Thermo Fisher Scientific) coupled to an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific). Peptides were loaded onto a trap column (Acclaim PepMap100, PN:164564, Thermo Fisher Scientific) in buffer A (0.1% formic acid) and separated on an analytical column (nanoESI column, 15 cm × 75 µm, NTCC-360/75-3-155, Nikkyo Technos, Tokyo, Japan) maintained at 35°C. Separation was performed at 300 nL/min using a linear gradient from 4% to 40% buffer B (100% acetonitrile, 0.1% formic acid) over 100 min, followed by a 1 min increase to 90% buffer B and a 5 min wash at 90% buffer B. Mass spectrometry was performed using data-dependent acquisition. Detailed MS acquisition parameters are summarized in Table S10. Raw data were processed using Proteome Discoverer Software 2.5 (Thermo Fisher Scientific) against the UniProt human proteome database (UP000005640; http://UniProt.org). Peptide-spectrum matches were validated using Percolator, and protein identifications were filtered at a false discovery rate of <1%. Label-free quantification was performed based on precursor ion intensities.
HostingRepository	jPOST
AnnounceDate	2026-09-19
AnnouncementXML	Submission_2026-09-18_10:14:32.239.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Takashi Hayashi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-18 19:23:48	ID requested
⏵ 1	2026-09-18 10:14:32	announced

Dataset with its publication pending

submitter keyword: megakaryocyte, imMKCL, artificial platelets

Hideki Hayashi
lab head
Takashi Hayashi
contact affiliation	Otsuka Pharmaceutical Co., Ltd.
dataset submitter

jPOST dataset URI

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST004857/