The study performed IP-MS using GBC-SD cells overexpressing STK31, immunoprecipitating its protein complexes with an anti-STK31 antibody and identifying interacting proteins via timsTOF Pro 2 mass spectrometry. Subsequently, the IP-MS results were integrated with phosphorylated proteome data to identify potential direct target proteins of STK31, among which c-Myc and FOXP1 were further validated by Co-IP/WB.