FAK (focal adhesion kinase) is a key regulator of cell adhesion and signaling. To identify potential FAK-interacting proteins, we expressed FLAG-tagged FAK (FAK-FLAG) or empty vector (EV) control in HEK293T cells, followed by anti-FLAG affinity pull-down. The immunoprecipitated complexes were resolved by SDS-PAGE and visualized by silver staining. Specific protein bands enriched in the FAK-FLAG lane compared to the EV control were excised, subjected to in‑gel trypsin digestion, and analyzed by LC‑MS/MS. Protein identification was performed, revealing their potential association with FAK. This dataset provides the raw mass spectrometry data and identification results for these candidate interactors.