During human infection, Cryptococcus neoformans can disseminate to the central nervous system causing often fatal cryptococcal meningitis in individuals with compromised immunity. To acutely adapt to host microenvironments, C. neoformans employs mechanisms to reprogram its translatome to provide cells with tools to survive the stressors of the human host. The identities and functions of ribosome-associating factors that may modulate this process in C. neoformans remain unknown. We hypothesized that following host-related stressors, specific proteins associate with ribosomes and influence stress adaptation in C. neoformans. Utilizing a proteomic approach, termed RiboPROT, to analyze the protein composition of mRNA-associated ribosomes from cells grown unstressed (30˚C), thermally stressed (shifted to 37°C), and oxidatively stressed (shifted to 2 mM H2O2) conditions. We identified unique factors enriched with actively translating ribosomes during host-related stressors