10,000 intensity) every 10 minutes throughout the LC gradient. The parameters used were as follows: Number of peptides per protein, 6; Number of transitions per peptide, 6; Peptide confidence threshold, 99%; False discovery rate threshold, 1%; exclude modified peptides, checked; XIC extraction window, 10 min, and; XIC width, 50 ppm. Protein peak areas were exported to Markerview (AB Sciex, Foster City, CA, USA) for further processing. The reversed and common contaminants hits were removed and then the protein abundance quantification table exported as a .tsv file. The filtered protein abundance table was exported as a .tsv file and then quantile-normalized using NormalyzerDE version 1.3.4 (https://normalyzerde.serve.scilifelab.se/). For all univariate and multivariate statistical analyses, the quantitative table was imputed for missing values (one-fifth minimum value imputation) and log2-transformed.]]>