Label-free vacuolar proteomics comparing wild-type and vps35Δ Saccharomyces cerevisiae cells harvested at stationary phase, aiming to identify retromer cargoes that accumulate on vacuoles upon retromer dysfunction and promote vacuolar membrane curvature associated with lipid droplet degradation. Data were acquired in parallel on both Orbitrap Eclipse and timsTOF Pro mass spectrometers for cross-validation.