We applied label-free quantitative proteomics to systematically profile the exosomal proteome under lithogenic conditions. After stimulating NRK-52E cells with 200 μg/mL COM for 24 hours, exosomes were isolated from the supernatant and designated as COM-Exo. Control exosomes (Ctrl-Exo) were similarly isolated from untreated NRK-52E cells. Three Ctrl-Exo samples and three COM-Exo samples were processed and subjected to label-free quantitative proteomics.