This project aims to investigate whether doxycycline (DOX) can attenuate TGF-β1-induced fibrotic activation in human hepatic stellate LX-2 cells. We performed quantitative proteomic analysis using LC-MS/MS to compare protein expression profiles between TGF-β1-treated and TGF-β1 plus DOX-treated groups. The results will reveal potential anti-fibrotic mechanisms of DOX and identify key proteins and pathways involved in the regulation of hepatic fibrosis.