Metabolic dysfunction-associated steatohepatitis (MASH) is driven by hepatocellular lipid overload and altered inter-organelle crosstalk. To elucidate the molecular mechanisms by which Perilipin 2 (PLIN2) regulates lipid droplet (LD)-mitochondria tethering, and to determine how this is affected by its O-GlcNAcylation under lipotoxic stress, we performed immunoprecipitation coupled with mass spectrometry (IP-MS)