To elucidate the molecular basis underlying sexual dimorphism in Tetranychus cinnabarinus, we conducted a systematic comparative analysis of protein expression profiles between male and female adults using high-throughput quantitative proteomics. Our results revealed significant proteomic differences between the two sexes, with differentially expressed proteins predominantly enriched in biological pathways associated with reproductive development, energy metabolism, and detoxification enzyme systems. These findings provide important proteomic data to support further understanding of the regulatory mechanisms governing sexual dimorphism in spider mites.