Mechanical pressure can substantially alter the mechano-microenvironment of corneal epithelial cells and activate the mechanosensitive channel Piezo1 as well as its downstream interacting proteins. In this study, we will perform immunoprecipitation using an anti-Piezo1 antibody, coupled with mass spectrometry analysis (IP-MS), to systematically identify the differential interactome of Piezo1 in corneal epithelial cells under pressure stimulation, with the aim of revealing the key molecular mechanisms underlying mechanotransduction.