We generated mice with a liver stellate cell-specific knockout of Prpf19, following 6 weeks of CCl₄ induction.The primary hepatic stellate cells were isolated from the liver for DIA-based quantitative proteomics analysis( prpf19 flox/flox =6/group; prpf19 flox/flox; LratERT2 = 6/group), thereby identifying proteins differentially expressed. The aim was to assess changes in the activation levels of primary liver stellate cells in these mice following Prpf19 knockout and to elucidate the potential regulatory mechanisms of Prpf19.