To elucidate the molecular mechanisms underlying the maintenance of dermal stem cells (DSCs), we performed a comprehensive, comparative proteomic analysis of primary dermal cells derived from Japanese individuals. Subjects were classified into two groups: DSC-rich (M1, M2, M3) and DSC-poor (L1, L3, L4, L5, L6). Primary dermal cells isolated from each donor were subjected to LC-MS/MS analysis to characterize and quantify their proteomic profiles. By comparing the proteomes of DSC-rich and DSC-poor cells, we aim to uncover key regulatory proteins and signaling pathways involved in DSC stemness and maintenance. These data will provide a valuable resource for understanding human skin homeostasis and developing novel skin regenerative strategies. (Note: The uploaded search result file contains data from additional samples belonging to another project; however, only the 8 Raw files relevant to this specific study have been uploaded and mapped.)