This study aims to elucidate the mechanism by which the long non-coding RNA SNHG26 promotes fibroblast activation in keloid pathogenesis through interactions with protein partners. Using RNA pull-down coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS), we identified proteins that specifically bind to SNHG26 in primary human keloid-derived fibroblasts. Two independent controls—biotinylated poly(A)25 RNA and a biotinylated random nucleotide sequence—were used to ensure binding specificity. The resulting dataset provides a resource for understanding SNHG26-mediated regulatory mechanisms in fibroblast activation and keloid development.