This project aims to identify Plk1-PBD interactors potentially involved in DNA repair. We performed unbiased proteomic screens in S and G2 phases. HeLa cells were synchronized by double-thymidine block-and-release, and pull-downs were conducted with GST-Plk1 PBD as bait. GST alone and a phospho-binding-deficient GST-PBD mutant (H538A/K540M) served as negative controls. Proteins were then analyzed by mass spectrometry.