This project investigates the interactome of MIRO2 using co-immunoprecipitation (Co-IP) followed by mass spectrometry analysis. Control and MIRO2-FLAG overexpressing cells were subjected to Co-IP to identify proteins that interact with MIRO2 and potentially facilitate mitochondrial-derived vesicle (MDV) trafficking to lysosomes under hypoxia/reoxygenation (H/R) stress. The dataset provides insights into MIRO2-associated protein complexes and their roles in lysosomal renewal, MDV formation, and organelle quality control.