Lipid extracts, specifically from marine microalgae Nannochloropsis oceanica (N.o.) and freshwater microalgae Chlorococcum amblystomatis (C.a.) in this research proposal, offer a great potential in the development of new pharmacotherapeutic applications against skin disease, accompanied by oxidative stress and associated chronic inflammation, and even skin cancer, due to their potential antioxidant, anti-inflammatory and anti-cancer properties reported in the literature. Therefore, lipid extracts obtained from two species of microalgae of different origins, characterized by a high content of bioactive compounds of a lipophilic nature, may also have cytoprotective effects through the modification of antioxidant and anti-inflammatory proteins, and ultimately the associated alterations in the intracellular molecular signaling via the changes in both associated protein expression, structure, and functionality. Thus, a proteomic research, using treatment with lipid extracts obtained from N.o. and C.a., has been planned to perform for analyzing the changes in the whole proteomic profile of human skin fibroblasts – in which its proteome is known to be dramatically disturbed by UVA radiation and plays a critical role in the intracellular signal transduction regarding important biological processes, such as the redox-dependent inflammatory response – under oxidative stress condition resulted by UVA irradiation. In this research, the proteins involved in the regulation of redox balance and inflammatory response will be particularly analyzed to understand the molecular mechanism behind the cytoprotective action of these microalgae lipid extracts. Moreover, a pull-down immunoprecipitation assay-combined proteomic analysis showing the human caspase-1 protein interactome area (associated with NLRP1, NLRP3, NLRC4, and AIM2 complexes) will be performed to understand the effect of these lipid extracts on the changes in the interaction between the intracellular redox signaling and inflammasome activities. The obtained proteomic data will indicate the regenerative/protective effect of lipid extracts on the protein homeostasis of fibroblasts exposed to UVA radiation, analyzed by assessing the molecular activity underlying the potential antioxidant and/or anti-inflammatory effects of these extracts. Also, analysis of the interaction area of inflammasome complexes may demonstrate the influence of these lipid extracts on the innate immune response of skin fibroblasts, which closely interacts with redox metabolism. This is another important problem in the development of effective antioxidant therapies, the limitations and failures of which are highlighted in clinical trials. Furthermore, the proteomic approach is a privileged analytical tool enabling knowledge and understanding of the communication between intracellular metabolic changes resulting from the interaction of oxidative stress and inflammatory protein networks in response to physicochemical extracellular factors.