Co-infection of human papillomavirus (HPV) and human immunodeficiency virus type 1 (HIV-1) in women have a six-fold higher risk of developing cervical cancer compared to those without HIV. To evaluate how paracrine signals from HIV-infected T-cells remodeled the proteome of cervical epithelial cells in culture, primary CD4+ T cells isolated from PBMC-enriched leukapheresis products (leukopaks) from two healthy donors were infected or uninfected with a replication-competent pNL4-3 HIV-1 strain for 72 hours. Secretome from the CD4+ T cell cultures was used to stimulate the human HPV-negative cervical epithelial cell line, C33A, for 72 hours. Then, C33A cells were harvested, and cell lysates were digested and subjected to global quantitative mass spectrometry (MS) based abundance proteomics and phosphoproteomics analyses. Both proteomics and phosphoproteomics outputs were analysed using bioinformatics approaches. These datasets revealed altered expression of proteins in the MAPK, PI3K-AKT, and β-catenin signaling pathways. Additionally, MS phosphoproteomics analysis confirmed PI3K-AKT pathway activation in cervical cells exposed to conditioned media from HIV-1-infected T cells.