PXD077692

PXD077692 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Heat shock protein family D member 1 (HSPD1) sustains ribosome biogenesis and protein synthesis as a component of its oncogenic function in lung adenocarcinoma
Description	Lung adenocarcinoma (LUAD) is the most prevalent histological subtype of lung cancer. Heat shock protein family D member 1 (HSPD1 or HSP60), a multifunctional chaperone, has been implicated in promoting lung cancer progression by regulating tumor cell growth, cancer-associated fibroblast activation, and angiogenesis. Despite these roles, the molecular mechanisms underlying its oncogenic activity remain incompletely understood. In this study, we conducted a proteomic analysis and functional investigations on HSPD1-knockdown and control A549 cells. Knockdown of HSPD1 suppressed cell proliferation, disrupted cell-cycle progression, and had no significant effect on apoptosis. Additionally, HSPD1-knockdown cells exhibited reduced colony formation, migration, and invasion capabilities. SWATH-targeted proteomics revealed 21 significantly altered proteins, mainly involved in ribosome-related functions and associated with overall survival in LUAD patients. Reduced levels of ribosomal proteins and translational capacity were further confirmed by Western blot and protein synthesis assays. A decrease in ribosome abundance in HSPD1-knockdown cells was also observed under transmission electron microscopy. HSPD1 knockdown also sensitized cells to homoharringtonine, a ribosomal-targeting chemotherapeutic. Collectively, these findings indicate that HSPD1 acts as an oncogenic driver in LUAD by modulating ribosome-related pathways and protein synthesis.
HostingRepository	jPOST
AnnounceDate	2026-04-28
AnnouncementXML	Submission_2026-04-27_19:40:53.623.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dr.Wararat Chiangjong
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	TripleTOF 6600+

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-27 19:40:50	ID requested
⏵ 1	2026-04-27 19:40:54	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: HSPD1, HSP60, shRNA knockdown, LUAD, ribosome, translational regulation, quantitative proteomics

submitter keyword: HSPD1, HSP60, shRNA knockdown, LUAD, ribosome, translational regulation, quantitative proteomics

Contact List

Siripat Aluksanasuwan
lab head
Dr.Wararat Chiangjong
contact affiliation	Mahidol University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST004583/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST004583/