PXD077563 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Convergent evolution of metabolic regulation governs redox adaptation in Toxoplasma |
| Description | This dataset comprises whole-proteome quantitative mass spectrometry analyses using TMT-16plex labeling to compare Toxoplasma gondii parasites expressing wild-type (cWT) or RNA-binding-deficient mutant (cMUT) forms of TgPRO — a CCCH-type zinc finger RNA-binding protein — grown under ambient (21%) and reduced (1%) oxygen conditions. TgPRO was identified in a genome-wide CRISPR screen as required for parasite fitness at high density, and loss of TgPRO selectively impairs parasite fitness at high density under ambient oxygen conditions, accompanied by elevated reactive oxygen species, mitochondrial dysfunction, and broad metabolic dysregulation. This proteomic dataset provides an unbiased view of the protein-level changes driven by TgPRO activity and oxygen availability, supporting the role of TgPRO in regulating transcripts involved in iron-sulfur cluster assembly and carbon metabolism, and can be further utilized to explore additional TgPRO-dependent targets and the broader proteomic response of T. gondii to hypoxia. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-14 |
| AnnouncementXML | Submission_2026-09-13_20:11:11.441.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD077563 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Chinmay Kalluraya |
| SpeciesList | scientific name: Toxoplasma gondii GT1; NCBI TaxID: NEWT:507601; |
| ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-04-23 21:34:02 | ID requested | |
| ⏵ 1 | 2026-09-13 20:11:12 | announced | |
Publication List
| 10.1016/j.cell.2026.07.029; |
| Giuliano CJ, Kalluraya CA, Kloehn J, Sloan MA, Bunkofske ME, Hunter CA, Soldati-Favre D, Harding CR, Lourido S, Convergent evolution of metabolic regulation governs redox adaptation in Toxoplasma. Cell, 189(20):6338-6355.e10(2026) [pubmed] |
| 10.6019/PXD077563; |
Keyword List
| submitter keyword: CRISPR screen, Post-transcriptional regulation,Toxoplasma gondii, NAD metabolism, Redox homeostasis, RNA-binding protein |
Contact List
| Sebastian Lourido |
|---|
| contact affiliation | Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Biology, Cambridge, MA, USA |
| contact email | lourido@wi.mit.edu |
| lab head | |
| Chinmay Kalluraya |
|---|
| contact affiliation | Whitehead Institute |
| contact email | ckallur@wi.mit.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD077563 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD077563
- Label: PRIDE project
- Name: Convergent evolution of metabolic regulation governs redox adaptation in Toxoplasma
