PXD076715

PXD076715 is an original dataset announced via ProteomeXchange.

Title	RpS12-mediated induction of the Xrp1short isoform links ribosomal protein mutations to cell competition
Description	Cell competition, the context-dependent cell elimination via short-range cell-cell interactions, was originally described in Drosophila, where heterozygous ribosomal protein (Rp+/-) mutant cells are outcompeted by wild-type neighbors. The transcription factor Xrp1 mediates most Rp+/- phenotypes, including reduced translation and competitiveness, yet how RpS12 induces Xrp1 has remained unclear. We show that RpS12 promotes a splice variant of Xrp1 encoding the short isoform Xrp1short, which defines the Rp+/- loser identity. RpS12 overexpression is sufficient to induce Xrp1short and a loser phenotype. Expression of either Xrp1 isoform alone can trigger competition, establishing Xrp1 as the central driver of the loser fate. When loser cells exceed a critical threshold, a quorum-like response supports their survival by post-transcriptionally downregulating Xrp1. We further identify the RNA-binding protein Syncrip, reduced in Rp+/- cells, as an Xrp1 suppressor whose depletion activates Xrp1-dependent competition. Thus, RpS12-dependent Xrp1short expression emerges as the primary Rp+/- signal driving cell competition.
HostingRepository	PRIDE
AnnounceDate	2026-05-16
AnnouncementXML	Submission_2026-05-16_07:52:48.128.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martina Samiotaki
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: NEWT:7227;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-07 16:36:33	ID requested
⏵ 1	2026-05-16 07:52:48	announced

Dataset with its publication pending

submitter keyword: Xrp1, Minutes, Drosophila,Ribosomal mutants

Marianthi Kiparaki
contact affiliation	Institute for Fundamental Biomedical Research Biomedical Sciences Research Centre, "Alexander Fleming” 34 Fleming str, 16672, Vari Greece
contact email	kiparaki@fleming.gr
lab head
Martina Samiotaki
contact affiliation	Protein Analysis Laboratory B.S.R.C. "Alexander Fleming", Alexander Fleming Street 34 16672, Vari, Greece
contact email	samiotaki@fleming.gr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD076715

PRIDE project URI

• PRIDE
  1. PXD076715
     1. Label: PRIDE project
     2. Name: RpS12-mediated induction of the Xrp1short isoform links ribosomal protein mutations to cell competition