This project includes two parts of mass spectrometry-based omics analysis. First, peptidomics analysis was performed to identify endogenous free peptides from Ophiocordyceps sinensis. Second, quantitative proteomic analysis was applied to investigate the global protein expression changes in RAW 264.7 macrophage cells treated with the immunomodulatory peptide WALGT identified from Ophiocordyceps sinensis.The dataset contains raw mass spectrometry data.