PXD076477 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | ProjDIA: Fragment-Bin Projection Scoring and Calibrated Confidence for Direct Infusion DIA Proteomics |
| Description | Direct-infusion shotgun proteome analysis (DI-SPA) increases throughput by removing liquid chromatography, but the resulting DIA spectra are highly multiplexed and difficult to interpret. This dataset was generated to evaluate ProjDIA, a computational workflow for direct-infusion DIA proteomics. ProjDIA uses fragment-bin projection scoring, confidence-guided two-pass mass-error recalibration, monotone q-value reporting, and semi-supervised rescoring to improve peptide and protein identification confidence. The dataset includes benchmarking experiments across MS2 resolution and sample load, replicate-series analyses, species-entrapment controls for external error assessment, and a three-species mixture for label-free quantification evaluation. The submission contains the raw mass spectrometry files together with processed search and result files used to support the analyses reported in the study. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-02 |
| AnnouncementXML | Submission_2026-04-02_13:05:44.076.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Herbi Yuliantoro |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-04-01 18:00:42 | ID requested | |
| ⏵ 1 | 2026-04-02 13:05:44 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: MCF7, proteomics, ProjDIA,Direct infusion, DIA, species entrapment, DI-SPA, HeLa, label-free quantification |
Contact List
| Peng Li |
|---|
| contact affiliation | Lehigh University |
| contact email | pel624@lehigh.edu |
| lab head | |
| Herbi Yuliantoro |
|---|
| contact affiliation | Lehigh University |
| contact email | hey325@lehigh.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD076477 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD076477
- Label: PRIDE project
- Name: ProjDIA: Fragment-Bin Projection Scoring and Calibrated Confidence for Direct Infusion DIA Proteomics
