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PXD076446
PXD076446 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | RGD-mediated biomimetic dynamic matrix stiffness signaling enable rapid 3D organoid formation |
| Description | Stiffness of extracellular matrix (ECM) is crucial for regulating tissue morphogenesis and cellular behavior. The Arg-Gly-Asp (RGD) motif is a key integrin ligand. However, whether RGD regulates cellular function and tissue remodeling through mechanical stiffness-driven mechanical signaling remains unclear. Here, we develop an easily prepared, ECM-free RGD-functionalized carboxymethylcellulose (CMC) hydrogel. By modulating its mechanical strength, the synergistic effect between RGD-mediated integrin linking and matrix stiffness regulates the formation and expansion of a wide range of tissue-specific organoids through the mechanical transduction pathway of integrin β1/FAK/YAP. Finally, using this method, we develop an acral melanoma organoid model that simulates its development driven by mechanical force and provides a platform for drug screening. These results highlight that RGD-functionalized, stiffness-tunable hydrogel is an excellent biomimetic model for investigating mechanotransduction-driven cell development and disease progression, providing a low-cost strategy for future high-throughput drug screening using organoids. |
| HostingRepository | iProX |
| AnnounceDate | 2026-04-01 |
| AnnouncementXML | Submission_2026-04-07_00:21:27.309.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yi Xie |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | acetylated residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-04-01 01:50:10 | ID requested | |
| ⏵ 1 | 2026-04-07 00:21:27 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: RGD-functionalized hydrogel, cell organoid, adjustable stiffness, acral melanoma, drug screening |
Contact List
| Ling Leng | |
|---|---|
| contact affiliation | Peking Union Medical College Hospital |
| contact email | lengling@pumch.cn |
| lab head | |
| Yi Xie | |
| contact affiliation | Peking Union Medical College Hospital |
| contact email | 18367700684@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
