Spinal cord injury (SCI) is a devastating and highly heterogeneous condition that often leads to permanent sensory, motor, and autonomic impairments. Beyond the initial mechanical trauma, a cascade of secondary biochemical events further amplifies tissue damage and functional loss. In this study, we investigate the proteomic consequences of the reactive aldehyde acrolein within the context of secondary injury pathology. Using comparative proteomic analyses, we evaluate biomolecular changes in SCI vs sham injury and acrolein vs saline injection models. We then apply integrated computational approaches to identify shared protein alterations and biological pathways that may mechanistically link acrolein exposure to SCI pathology.