Proteomic profiling was performed by Novogene (Beijing, China), using data-independent acquisition (DIA) proteomics on paired bone marrow aspirate samples from 5 non-M3 AML patients before and after VD-CAG treatment. All patients achieved composite complete response (CRc) after 1 cycle of VD-CAG induction. Protein extraction, quantification, enzymatic digestion with trypsin (allowing up to 2 missed cleavage sites), desalting, and fractionation were conducted following standard protocols to ensure data quality at each step. Peptides were separated using nano-LC on a Vanquish Neo system and analyzed on an Orbitrap Astral high-resolution mass spectrometer, which features a quadrupole for precursor ion selection, an Orbitrap analyzer for high-dynamic-range HRAM spectra, and an Astral analyzer for high-sensitivity, up to 200 Hz HRAM spectral acquisition.