PXD075464-1

PXD075464 is an original dataset announced via ProteomeXchange.

Title	A novel player in trypanosome endoplasmic reticulum quality control with apparent similarities to the mammalian BiP nucleotide exchange inhibitor MANF
Description	We have identified the saponin-domain containing protein Tb927.5.1160 as a novel endoplasmic reticulum (ER) factor in Trypanosoma brucei. Tb927.5.1160 acts proximal to the prototypic ER chaperon BiP, the conserved heat shock protein 70 family chaperon playing a key role in protein folding and ER quality control, and exhibits a conserved BiP binding region shared with the mammalian BiP nucleotide exchange inhibitor MANF (mesencephalic astrocyte-derived neurotrophic factor). We demonstrated by inducible RNAi depletion and over-expression that Tb927.5.1160 has a marked effect on the sensitivity toward ER-stress and is essential for T. brucei viability. Here we deposit whole cell proteomics data monitoring protein abundance changes after 48 hours induction of Tb927.5.1160 RNAi in bloodstream form (BSF) and procyclic-form (PCF) T. brucei, and Tb927.5.1160 overexpression in BSF.
HostingRepository	PRIDE
AnnounceDate	2026-04-05
AnnouncementXML	Submission_2026-04-05_02:00:29.278.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Zoltner
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: NEWT:5691;
ModificationList	carbamoylated residue
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-10 14:52:16	ID requested
⏵ 1	2026-04-05 02:00:29	announced

Dataset with its publication pending

submitter keyword: Trypanosoma bucei, endoplasmic reticulum, ERQC

Martin Zolther
contact affiliation	Department of Parasitology, Faculty of Science, Charles University in Prague, Biocev, Vestec, Prague, Czech Republic
contact email	zoltnerm@natur.cuni.cz
lab head
Martin Zoltner
contact affiliation	Division of Biological Chemistry & Drug Discovery School of Life Sciences University of Dundee Dundee DD1 5EH
contact email	m.zoltner@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD075464
     1. Label: PRIDE project
     2. Name: A novel player in trypanosome endoplasmic reticulum quality control with apparent similarities to the mammalian BiP nucleotide exchange inhibitor MANF